Journal: Cancers

Article Title: A Double-Negative Feedback Interaction between miR-21 and PPAR-α in Clear Renal Cell Carcinoma

doi: 10.3390/cancers14030795

Figure Lengend Snippet: miR-21 and PPAR-α expressions in HK-2 and renal cancer cell lines before confluence (BC) and two days after confluence (C + 2). ( A ) Using RT-qPCR, miR-21 expression was determined in the 786-O, ACHN, RCC10, and RCC4 renal cancer cells as compared to normal HK-2 renal epithelial cells. RNU48 was used as an internal control. The values are means ± SEM and represent at least three separate experiments (* p < 0.05, ** p < 0.01, and *** p < 0.001). ( B ) Western blotting was performed on whole cell extracts. Antibodies against PPAR-α and β-actin were used.

Article Snippet: The HK-2 human proximal tubule epithelial cell line, ACHN, and 786-O human renal cancer cell lines were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Quantitative RT-PCR, Expressing, Control, Western Blot

Journal: Cell Genomics

Article Title: Defining hypoxia in cancer: A landmark evaluation of hypoxia gene expression signatures

doi: 10.1016/j.xgen.2025.100764

Figure Lengend Snippet: Published hypoxia signatures assessed in this study

Article Snippet: 16417408 , Chi 2006 , in vitro , ECs, SMCs, HMECs, RPTECs , coronary artery endothelial cells, smooth muscle cells, mammalian epithelial cells, renal proximal tubule epithelial cells , 111 , 111 , 0.1–2% O 2 , 1–24 h , Chi et al. .

Techniques: In Vitro, Transformation Assay